A DEMONSTRATION OF ANTIBODY ACTIVITY ON MICROSOMES

Abstract

Guinea pigs were injected with 0.1 ml of 2,4-dinitrophenyl bovine gamma globulin (DNP-BgammaG) or bovine gamma globulin (BgammaG) in Freund''s adjuvant for a total of 1.2 mg protein. Three to 6 weeks after immunization microsome preparations were made from lymph nodes and liver. The assay for antibody activity on microsomes in these antigenically stimulated animals was based on the reversible binding of a soluble antigen by antibodies which were insoluble due to their association with microsomal particles. I131 labeled antigen was used to provide an indicator of high sensitivity. Antibody activity specific for the 2,4-dinitrophenyl group was demonstrated to be induced on the microsomes of regional lymph nodes which drain sites of injection of DNP-BgammaG. The possibility that the antibody activity was an artifact arising from adsorption of soluble antibody was excluded by a variety of experiments.