Cyanogen‐induced γ‐glutamyl to imidazole cross‐link in carbonic anhydrase
Open Access
- 2 December 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 193 (2), 145-149
- https://doi.org/10.1016/0014-5793(85)80139-3
Abstract
The irreversible inhibition of carbonic anhydrase by cyanogen occurs by a unique mechanism. Cyanogen is an affinity label: it behaves like a carbodiimide and produces an intra‐molecular cross‐link without being incorporated. The nucleophile‐labile cross‐link is formed between a γ‐COOH of a Glu and an imidazole of a His with a 1:1:1 stoichiometry with the enzyme. The deletion of ~ 1 Glu and ~ 1 His was noted by amino acid analysis of enzymatically hydrolyzed carbonic anhydrase. The modified Glu was converted to 2,4‐diaminobutanoic acid and quantitated by amino acid analysis. The presence and quantity of modified His was supported through high‐resolution proton NMR analysis.Keywords
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