Three‐dimensional reconstruction from images of tilted specimens: the paramyosin filament

Abstract

The 3-dimensional structure of the paramyosin filament from the adductor muscle of the oyster Ostrea edulis was reconstructed from images obtained when the specimen is tilted at different angles about its long axis. The micrographs were correlated spatially using gold marker beads and digitized. The different views contained in each micrograph were combined using a computational technique involving Fourier transforms and the resulting 3-dimensional images are presented as various sets of serial sections. The Bear-Selby net can be seen at all levels in the specimen.