ANALYSIS OF A SEROLOGICAL DETERMINANT OF H‐Y ANTIGEN: EVIDENCE FOR CARBOHYDRATE SPECIFICITY USING AN H‐Y SPECIFIC MONOCLONAL ANTIBODY

Abstract

A monoclonal antibody to [mouse] H-Y antigen was used to characterize the molecular determinant responsible for H-Y reactivity. H-Y-positive cells were treated with enzymes which alter carbohydrate structure and then were tested for their ability to absorb monoclonal anti-H-Y antibody. Indications are that the serological determinant recognized by this antibody is a glycoconjugate containing terminal non-reducing and internal galactosyl residues.