A T-lymphoma transmembrane glycoprotein (gp180) is linked to the cytoskeletal protein, fodrin.
Open Access
- 1 August 1985
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 101 (2), 477-487
- https://doi.org/10.1083/jcb.101.2.477
Abstract
A major mouse T-lymphoma surface glycoprotein (gp180) has been identified by labeling cells with 125I and [3H]glucosamine. After ligand-induced receptor patching and/or capping, the amount of gp 180 in the membrane-associated cytoskeleton fraction increases in direct proportion to the percentage of patched/capped cells. There is a parallel increase in the amount of fodrin in the membrane-associated cytoskeleton fraction. Evidence is presented that gp180 is the same as or very similar to the T-lymphocyte-specific glycoprotein T-200. An immunobinding assay of Nonidet P-40-solubilized plasma membrane selectively co-isolates gp180 and fodrin. After induction of receptor rearrangement, double-label immunofluorescence reveals that fodrin accumulated directly beneath gp180 patches and caps. Membrane extraction with Triton X-114 followed by sucrose gradient centrifugation permits isolation of a gp180-fodrin complex with a 1:1 molar ratio and sedimentation coefficient(s) of approximately 20. This complex remains stable during isoelectric focusing and exhibits a pl in the range of 5.2-5.7. On the basis of our results we conclude that gp180, an integral membrane glycoprotein, and fodrin, a component of the membrane-associated cytoskeleton, are closely associated into a complex. Furthermore, we contend that, through fodrin's association with actin, this complex is of functional significance in ligand-induced patching and capping of gp180. We also propose that, through lateral interactions in the plane of the membrane, the gp180-fodrin complex might be responsible for linking other surface receptors to the intracellular microfilament network during lymphocyte patching and capping.This publication has 52 references indexed in Scilit:
- Lectin-binding and spontaneous capping characteristics of the thymocyte glycophorin-like glycoproteinExperimental Cell Research, 1984
- Receptor capping in mouse T-lymphoma cells: A Ca2+ and calmodulin-stimulated ATP-dependent processThe Journal of Membrane Biology, 1983
- A protein immunologically related to erythrocyte band 4.1 is found on stress fibres of non-erythroid cellsNature, 1982
- Transmembrane linkage between surface glycoproteins and components of the cytoplasm in neutrophil leukocytes.The Journal of cell biology, 1981
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Purification of deoxyribonucleic acid polymerase .delta. from calf thymus: partial characterization of physical propertiesBiochemistry, 1980
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Influence of cholera toxin on the regulation of adenylate cyclase by GTPBiochemical and Biophysical Research Communications, 1977
- The potential of Ultrogel®, an agarose‐polyacrylamide copolymer, as a matrix for affinity chromatographyFEBS Letters, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970