Release of viral hemagglutinin and nucleocapsid components after disruption of Sindbis virus with ether

Abstract

Purified Sindbis virus is split into two components upon treatment with ether. These are (a) a hemagglutinin which sediments at 55 S and is non-infectious. This fraction was shown to be devoid of phospholipid and to contain the envelope structural protein of the virion; (b) a fraction sedimenting at 135 S, which is devoid of RNA, but contains viral nucleocapsid structural protein. This fraction may represent the RNA-less nucleocapsid of the virion.