Microneutralization test for influenza A and B and parainfluenza 1 and 2 viruses that uses continuous cell lines and fresh serum enhancement
- 1 September 1980
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 12 (3), 426-432
- https://doi.org/10.1128/jcm.12.3.426-432.1980
Abstract
A modified microneutralization test for influenza A and B and parainfluenza 1 and 2 viruses was developed. Use of continuous cell lines (Madin-Darby canine kidney and LLC-MK2), hemagglutination for virus detection, and transfer plates and other microtiter equipment resulted in a highly mechanized technique suitable for titrating large numbers of sera easily and relatively inexpensively. Titers of heat-inactivated human sera were enhanced 0.5-3.5 log2 by addition of fresh hamster or human serum to the test. Seroconversion rates and identification of seronegative persons were not changed by fresh serum enhancement, but the magnitude of seroconversion was often increased and the distribution of positive titers was broadened. For influenza A and B, seroconversion rates in the microneutralization test were equivalent to those obtained in rhesus monkey kidney tube neutralization tests. For influenza B, seroconversion rates by microneutralization were almost double those obtained with the hemagglutination inhibition test, but the rates were similar for influenza A/USSR (H1N1). Preexisting microneutralization titers correlated well with resistance to infection with influenza B. Limited experience with parainfluenza 1 and 2 was similar to previously reported findings with the tube neutralization test.This publication has 16 references indexed in Scilit:
- Comparison of Influenza B/Hong Kong Virus Infections among Infants, Children, and Young AdultsThe Journal of Infectious Diseases, 1980
- Reinfection with Influenza A (H3N2) Virus in Young Children and Their FamiliesThe Journal of Infectious Diseases, 1979
- Sensitive neutralization test for virus antibodyArchiv für die gesamte Virusforschung, 1978
- Neutralization of Animal VirusesPublished by Elsevier ,1978
- Comparative Efficacy of Neuraminidase-Specific and Conventional Influenza Virus Vaccines in Induction of Antibody to Neuraminidase in HumansThe Journal of Infectious Diseases, 1976
- Automated microtransfer technique for the assay of poliovirus- and mumps virus-neutralizing antibodies.1974
- Experimental respiratory syncytial virus infection of adults. Possible mechanisms of resistance to infection and illness.1971
- Piggy-back microtransfer technique.1969
- COMPLEMENT COMPONENTS REQUIRED FOR VIRUS NEUTRALIZATION BY EARLY IMMUNOGLOBULIN ANTIBODYProceedings of the National Academy of Sciences, 1969
- A New Micro-Neutralization Test for Antibody Determination and Typing of Parainfluenza and Influenza VirusesExperimental Biology and Medicine, 1967