The Testicular Receptor for Follicle Stimulating Hormone: Structure and Functional Expression of Cloned cDNA
- 1 April 1990
- journal article
- research article
- Published by The Endocrine Society in Molecular Endocrinology
- Vol. 4 (4), 525-530
- https://doi.org/10.1210/mend-4-4-525
Abstract
Cloned cDNA encoding the rat Sertoli cell receptor for FSH was isolated from a cognate library and functionally expressed cultured mammalian cells. The FSH receptor (FSH-R), as predicted from the cDNA, is a single 75K polypeptide with a 348 residue extracellular domain which contains three N-linked glycosylation sites. This domain is connected to a structure containing seven putative transmembrane segments which displays sequence similarity to G protein-coupled receptors. Thus, the FSH-R is identical in its structural design to the LH/CG receptors (LH/CG-R). Furthermore, both receptors display 50% sequence similarity in their large extracellular domains and 80% identity across the seven transmembrane segments. Expression of the cloned cDNA in mammalian cells conferred FHS-dependent cAMP accumulation. The selectivity for FSH is attested by the fact that the related human glycoprotein hormones human CG and human TSH do not stimulate adenylyl cyclase in FSH-R expressing cells even when these hormones are present at high concentrations.Keywords
This publication has 26 references indexed in Scilit:
- Identification of residues required for ligand binding to the beta-adrenergic receptor.Proceedings of the National Academy of Sciences, 1987
- Quaternary structure of the calf testis follitropin receptor.Journal of Biological Chemistry, 1986
- Beta-adrenergic receptor kinase: identification of a novel protein kinase that phosphorylates the agonist-occupied form of the receptor.Proceedings of the National Academy of Sciences, 1986
- The subunit structure of the follitropin (FSH) receptor. Photoaffinity labeling of the membrane-bound receptor follitropin complex in situ.Journal of Biological Chemistry, 1985
- Photoaffinity labeling of the follitropin receptor.Journal of Biological Chemistry, 1985
- Composition of cross-linked 125I-follitropin-receptor complexes.Journal of Biological Chemistry, 1985
- Intersubunit disulfides of the follitropin receptor.Journal of Biological Chemistry, 1985
- A Hypothesis for the Role of Dithiol‐Disulfide Interchange in Solute Transport and Energy‐Transducing ProcessesEuropean Journal of Biochemistry, 1982
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Ovarian Follicular Development in the Rat: Hormone Receptor Regulation by Estradiol, Follicle Stimulating Hormone and Luteinizing Hormone1Endocrinology, 1976