Tetraenoic Species Are Conserved in Muscarinically Enhanced Inositide Turnover
- 1 February 1985
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (2), 540-543
- https://doi.org/10.1111/j.1471-4159.1985.tb05446.x
Abstract
Carbamylcholine enhances the labeling of phosphatidate and phosphatidylinositol from 32 P i in nerve endings. Approximately 74% of labeled phosphatidate and 85% of labeled phosphatidylinositol produced on muscarinic stimulation are accounted for by tetraenoic species, as detected by argentation TLC. Incubation of membranes derived from nerve endings with [Γ- 32 P]ATP under conditions of phosphodiesteratic degradation of endogenous polyphosphoinositides resulted in increased labeling of phosphatidate. Approximately 78% of the newly formed phosphatidate was in a tetraenoic fraction. It is concluded that in muscarinically stimulated nerve endings, the diacylglycerol moiety is conserved following diacylglycerol release from polyphosphoinositides through its resynthesis to inositol lipid via phosphatidateKeywords
This publication has 21 references indexed in Scilit:
- Renewed interest in the polyphosphoinositidesTrends in Biochemical Sciences, 1984
- Phosphodiesteratic breakdown of endogenous polyphosphoinositides in nerve ending membranesBiochemical and Biophysical Research Communications, 1983
- The phosphatidylinositol cycle and the regulation of arachidonic acid productionNature, 1981
- Effects of cholinergic stimulation on levels and fatty acid composition of diacylglycerols in mouse pancreasBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Phospholipid Metabolism in Stimulated Human PlateletsJCI Insight, 1980
- Origin of the arachidonic acid released post-mortem in rat forebrainBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
- Positional distribution and turnover of fatty acids in phosphatidic acid, phosphoinositides, phosphatidylcholine and phosphatidylethanolamine in rat brain in vivoBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972
- Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brainBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970
- Mono- and dimethyl phosphatidates from different subtypes of choline and ethanolamine glycerophosphatidesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- The fatty acid components of polyphosphoinositide prepared from calf brainBiochimica et Biophysica Acta, 1963