Bivalent Cholesterol-Based Coupling of Oligonucletides to Lipid Membrane Assemblies
- 1 August 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (33), 10224-10225
- https://doi.org/10.1021/ja048514b
Abstract
By mimicking Nature's way of utilizing multivalent interactions, we introduce in the present work a novel method to improve the strength of cholesterol-based DNA coupling to lipid membranes. The bivalent coupling of DNA was accomplished by hybridization between a 15-mer DNA and a 30-mer DNA, being modified with cholesterol in the 3' and 5' end, respectively. Compared with DNA modified with one cholesterol moiety only, the binding strength to lipid membranes appears to be significantly stronger and even irreversible over the time scale investigated (∼1 hr). First, this means that the bivalent coupling can be used to precisely control the number of DNA per lipid-membrane area. Second, the strong coupling is demonstrated to facilitate DNA-hybridization kinetics studies. Third, exchange of DNA between differently DNA-modified vesicles was demonstrated to be significantly reduced. The latter condition was verified via site-selective and sequence-specific sorting of differently DNA-modified lipid vesicles on a low-density cDNA array. This means of spatially control the location of different types of lipid vesicles is likely to find important applications in relation to the rapid progress currently made in the protein chip technology and the emerging need for efficient ways to develop membrane protein arrays.Keywords
This publication has 7 references indexed in Scilit:
- Patterns of DNA‐Labeled and scFv‐Antibody‐Carrying Lipid Vesicles Directed by Material‐Specific Immobilization of DNA and Supported Lipid Bilayer Formation on an Au/SiO2 TemplateChemBioChem, 2003
- Arrays of Mobile Tethered Vesicles on Supported Lipid BilayersJournal of the American Chemical Society, 2003
- Tools for the Biomolecular EngineerScience, 2002
- Protein microarrays: prospects and problemsChemistry & Biology, 2001
- Amplified Microgravimetric Quartz-Crystal-Microbalance Assay of DNA Using Oligonucleotide-Functionalized Liposomes or Biotinylated LiposomesJournal of the American Chemical Society, 1999
- Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and InhibitorsAngewandte Chemie International Edition, 1998
- Surface Specific Kinetics of Lipid Vesicle Adsorption Measured with a Quartz Crystal MicrobalanceBiophysical Journal, 1998