Identification of the N-ethylmaleimide reactive protein of the mitochondrial phosphate transporter
- 1 May 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (10), 2098-2102
- https://doi.org/10.1021/bi00577a040
Abstract
The mitochondrial phosphate carrier from Sarcophaga bullata thorax preparations is inhibited by the SH reagents p-(hydroxymercuri)benzoate [PHMB] and N-ethylmaleimide [NEM]. Based on an analysis utilizing dodecyl sulfate-polyacrylamide gels, an SH-containing 32,000 dalton protein was identified as a component of the phosphate carrier system. Two other 3H- NEM-labeled proteins of the inner mitochondrial membrane were eliminated from this role Wohlrab, and Greaney, on the basis that band IV (45,000 daltons) is absent from rat heart sonic submitochondrial particles and band VII (6500 daltons) does not react with PHMB. The mobility of the 32,000 dalton protein (0.43) is lower than that of the .gamma. subunit of the mitochondrial ATPase (0.46) and the carboxyatractyloside binding protein (0.48) on 12.5% dodecyl sulfate-polyacrylamide gels. In these flight muscle mitochondria, 0.87 nmol of 3H-NEM/nmol of cytochrome a is bound to the 32,000 dalton protein.This publication has 8 references indexed in Scilit:
- Mitochondrial phosphate transport and the N-ethylmaleimide binding proteins of the inner membraneBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1978
- Isolation of the ADP, ATP carrier as the carboxyatractylate · protein complex from mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1978
- Mitochondrial phosphate transport: Correlation between alkylation of membrane proteins with N-[3H]ethylmaleimide and inhibition of transportBiochemical and Biophysical Research Communications, 1978
- Phosphate transport in yeast mitochondria: purification and characterization of a mitoribosomal synthesis dependent proteolipid showing a high affinity for phosphateBiochemistry, 1978
- Stimulation of adenine nucleotide translocation in reconstituted vesicles by phosphate and the phosphate transporterBiochemical and Biophysical Research Communications, 1977
- Purification and reconstitution of the phosphate transporter from bovine heart mitochondriaBiochemical and Biophysical Research Communications, 1977
- Identification of a Membrane Protein Involved in Mitochondrial Phosphate TransportEuropean Journal of Biochemistry, 1976
- GRAMICIDIN AND ION TRANSPORT IN ISOLATED LIVER MITOCHONDRIABiochemical Journal, 1965