GABAA receptors containing α1 and β2 subunits are mainly localized on neurons in the ventral pallidum
- 1 June 1991
- Vol. 8 (2), 75-85
- https://doi.org/10.1002/syn.890080202
Abstract
The γ-aminobutyric acid (GABA) projection from the nucleus accumbens to the ventral pallidum (VP) is important in the regulation of locomotion. Thus, stimulation and inhibition of GABAA receptors in the VP can alter locomotor activity. To determine whether the GABAA receptors are located presynaptically on accumbens efferents to the VP or postysnaptically on neurons intrinsic to the VP two experiments were performed. In the first, quinolinic acid lesions of the nucleus accumbens did not alter [3H]muscimol binding in the VP, while lesions in the VP significantly reduced (60–80%) binding as measured by light microscopic receptor autoradiography. In the second experiment, in situ hybridization with oligonucleotide probes for mRNAs of the α1 and β2 subunits of the GABAA receptor was examined in the nucleus accumbens and VP. No mRNA for either subunit was observed in the nucleus accumbens, although many positively labeled neurons were present within the VP. By contrast, a moderate to high density of cells in both the nucleus accumbens and VP contained mRNA for glutamic acid decarboxylase. These data argue that the majority of GABAA receptors in the VP are not located presynaptically on axonal terminals originating from neurons in the nucleus accumbens.Keywords
This publication has 67 references indexed in Scilit:
- Functional expression and sites of gene transcription of a novel α subunit of the GABAA receptor in rat brainFEBS Letters, 1990
- Pharmacological characterization and region‐specific expression in brain of the β2‐ and β3‐subunits of the rat GABAA receptorFEBS Letters, 1989
- Cloning and expression of a novel rat GABAA receptorFEBS Letters, 1989
- Locomotor hyperactivity in the rat after infusion of muscimol and [d-Ala2]Met-enkephalin into the nucleus basalis magnocellularis. Possible interaction with cortical cholinergic projectionsBrain Research, 1988
- Lesions of the dorsomedial nucleus of the thalamus, medial prefrontal cortex and pedunculopontine nucleus: effects on locomotor activity mediated by nucleus accumbens-ventral pallidal circuitryBrain Research, 1987
- [3H]Muscimol photolabels the γ-aminobutyric acid receptor binding site on a peptide subunit distinct from that labeled with benzodiazepinesBiochemical and Biophysical Research Communications, 1986
- Photoaffinity Labeling of the GABAA Receptor with [3H]MuscimolJournal of Neurochemistry, 1985
- The neural substrates of apomorphine-stimulated locomotor activity following denervation of the nucleus accumbensLife Sciences, 1984
- Organization of the efferent projections of the nucleus accumbens to pallidal, hypothalamic, and mesencephalic structures: A tracing and immunohistochemical study in the catJournal of Comparative Neurology, 1984
- Increase in Striatal [3H]Muscimol Binding Following Intrastriatal Injection of Kainic Acid: A Denervation Supersensitivity PhenomenonJournal of Neurochemistry, 1980