A Radiation-Resistant Rod-Shaped Bacterium, Deinobacter grandis gen. nov., sp. nov., with Peptidoglycan Containing Ornithine

Abstract

Four similar strains of gram-negative, red or pink, radiation-resistant, rod-shaped bacteria were isolated from animal feces and freshwater fish. Cell division was a simple type. The deoxyribonucleic acid guanine-plus-cytosine (G + C) base ratio was 69 mol%, and menaquinone (MK-8) was found in their respiratory chain. The predominant cellular fatty acids were C15:1 and C16:1. Ornithine was the diamino acid in the peptidoglycan, and the peptidoglycan type of a representative strain was the ornithine-glycine2 (A3.beta.) type. They stained gram negative (by the Hucker modification staining method), and they showed an unusual cell wall structure similar to that of the genus Deinococcus. Their chemotaxonomic features were also very close to those of Deinococcus species. The ribonuclease T1 catalog of 16S ribosomal ribonucleic acid showed a close relationship to those of Deinococcus species. It is proposed that they be named Deinobacter grandis gen. nov. sp. nov.; the designated type strain KS 0485 (= IAM 13005).