ISOLATION AND REGIONAL LOCALIZATION OF DNA SEGMENTS REVEALING POLYMORPHIC LOCI FROM HUMAN CHROMOSOME-13

Abstract

A recombinant DNA library enriched for portions of human chromosome 13 was constructed from a hamster-human somatic cell hybrid that contained human chromosomes 13, 12 and 6p. A total of 733 phages were identified that contain human DNA inserts, and 46 single-copy subfragments were derived and used as probes on Southern transfers of genomic DNA isolated from unrelated individuals. From this set, 9 fragments revealing polymorphic loci (RFLP) in Msp I- or Taq I-digested DNA were identified, of which 3 are polymorphic with both enzymes. Six of these probes segregated concordantly with human chromosome 13 in a somatic cell hybrid mapping panel, and the RFLP at these loci behaved as codominant Mendelian alleles. Additionally, hybridization to DNA isolated from cells containing various deletions of chromosome 13 has allowed regional localization. This recombinant DNA library will be useful in the study of retinoblastoma as well as in the study of the mechanisms responsible for abnormalities of this autosome.