Chitin Synthase in Aspergillus nidulans: Properties and Proteolytic Activation
- 1 March 1977
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 99 (1), 69-76
- https://doi.org/10.1099/00221287-99-1-69
Abstract
Chitin synthase (EC 2.4.1.16) from membrane preparations of A. nidulans was characterized and the optimum conditions for enzyme activity were determined. A reaction velocity-substrate concentration plot was sigmoidal, but was hyperbolic in the presence of N-acetylglucosamine when the Km for UDP-N-acetylglucosamine was 3.1 mmol l-1. Chitin synthase activity could be increased 6-fold by digestion of enzyme preparations with trypsin for short periods. The trypsin-activated enzyme had altered kinetic and storage properties.This publication has 8 references indexed in Scilit:
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