UPTAKE OF METHYLMERCURY AND INORGANIC MERCURY BY MOUSE GLIOMA AND MOUSE NEURO-BLASTOMA CELLS

Abstract

Methylmercury was taken up preferentially by mouse glioma and mouse neuroblastoma cells relative to inorganic Hg. Methylmercury uptake was depressed by lowering the cellular ATP level or the incubation temperature, while the uptake of inorganic Hg was not affected by these treatments. When the cells were treated with reagents such as cytochalasin B, colchicine and vinblastine known to affect membrane permeability, changes in permeability to methylmercury caused by these reagents were markedly different from those to inorganic Hg. Inorganic Hg > 2 .times. 10-5 M caused the release of 2-deoxyglucose trapped in the cells and the amount of inorganic Hg taken up by the cells increased markedly at higher concentrations. Inorganic Hg appeared to move into the cells after disrupting the membrane barrier, while methylmercury penetrated the cells without any noticeable damage to the barrier.