SECRET AGENT, anArabidopsis thaliana O-GlcNAc transferase, modifies thePlum pox viruscapsid protein
- 29 September 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (25), 5829-5835
- https://doi.org/10.1016/j.febslet.2006.09.046
Abstract
The capsid protein of Plum pox virus (PPV-CP) is modified with O-linked GlcNAc (O-GlcNAc). While Arabidopsis has two O-GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV-CP and that the modification plays a role in the infection process. Here, we show that when co-expressed in Escherichia coli SEC modifies PPV-CP. Deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coliKeywords
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