Signals regulating hepatitis B surface antigen transcription
- 1 September 1983
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 305 (5932), 336-338
- https://doi.org/10.1038/305336a0
Abstract
About 200 million people are chronic carriers of hepatitis B surface antigen (HBsAg), but since hepatitis B virus (HBV) cannot be propagated in vitro, HBsAg transcription has been studied only in cell lines containing HBV DNA integrated into chromosomes, and HBsAg-related mRNAs 2.0 to 2.5 kilobases (kb) long have been described1–4. We have analysed the transcripts produced in an infected chimpanzee liver and in a rat cell line containing HBV DNA5. In contrast to previous suppositions1,2 we report here that the major S gene transcript initiates close to the S gene, that is, within the ‘pre-S’ region6 and is processed/polyadenylated at a site situated within the core gene. The efficiency of processing/polyadenylation at this site varies between the chimpanzee liver and the rat cell line studied. The S gene promoter does not contain a TATA box but instead has a sequence homologous to that which positions the 5′ ends of the major simian virus 40 (SV40) late transcript7.Keywords
This publication has 30 references indexed in Scilit:
- Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector.Molecular and Cellular Biology, 1983
- Core and E antigen synthesis in rodent cells transformed with hepatitis B virus DNA is associated with greater than genome length viral messenger RNAsJournal of Molecular Biology, 1983
- Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNACell, 1982
- Expression of the hepatitis B virus surface, core and E antigen genes by stable rat and mouse cell linesJournal of Molecular Biology, 1982
- Cloned HBV DNA causes hepatitis in chimpanzeesNature, 1982
- Biology of Hepatitis B VirusScience, 1981
- Integration of hepatitis B virus sequences and their expression in a human hepatoma cellNature, 1980
- Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell lineNature, 1980
- Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coliNature, 1979
- Spliced early mRNAs of simian virus 40Proceedings of the National Academy of Sciences, 1978