Monoclonal Antibody Biosensor for Antigen Monitoring
- 1 November 1987
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 20 (11), 1781-1790
- https://doi.org/10.1080/00032718708082595
Abstract
A new class of fully reversible biosensors, utilizing a layer of monoclonal antibodies trapped at the sensing surface of an immobilized-antigen membrane, is shown to be suited for continuous monitoring of antigen levels. The model sensor described gives a potentiometric response to the hapten 2,4-dinitrophenol (DNP) at micromolar concentration levels through competitive interaction with monoclonal anti-DNP antibodies held within the sensor tip. The proposed design yields a reusable, reagentless antigen sensor with good analytical precision and an operating lifetime of at least 17 days.Keywords
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