Abstract
Treatment of D-fructose 1-phosphate with dicyclohexylcarbodi-imide in aqueous pyridine in the presence of triethylamine led to the formation of D-fructopyranose, 1,2-cyclic phosphate (II) and D-fructofuranose 1,2-cyclic phosphate (V). Alkaline hydrolysis of (n) and (V) gave D-fructopyranose 2-phosphate and D-fructofuranose 2-phosphate respectively, besides D-fructose 1-phosphate. The optical rotations of the synthetic D-fructose 2-phosphates suggest that they are the [beta]-anomers. The different D-fructose esters were shown to separate well by ion-exchange chromatography on Dowex 1 (borate form).