Structure of a Carbohydrate Moiety of a Unit A Glycopeptide of Calf Thyroglobulin1

Abstract

The structure of the carbohydrate moiety of a unit A glycopeptide of calf thyroglobulin was studied by glycosidase digestion, acetolysis, and methylation analysis. Based on the results, the whole structure of a unit A glycopeptide with the largest carbohydrate moiety was determined as Manαl → 2Manαl → 6(Manαl → 2Manαl → 3) →6(Manαl → 2Manα1 → 2manα1 → 3)Manβ → 4GlcNAcβ1 → 4GlcNAC → Asn-Peptide.