Über die Umwandlung der 3-Hydroxy-anthranilsäure in Chinolinsäure und Nicotinsäure im tierischen Organismus II. Die Isolierung und vorläufige Charakterisierung des primären Oxydationsproduktes der 3-Hydroxy-anthranilsäure

Abstract

The primary enzymatic cleavage product of 3-hydroxyanthranilic acid was isolated as 2,4-dinitrophenylhydrazone. The cleavage product is regarded as l-amino-4-formyl-l,3-butadien-l,2-dicarboxylic acid. Two substances were isolated in the form of crystalline dinitrophenylhydra-zones after mild hydrogenation of the primary enzymatic cleavage product. The UV absorption spectra show the typical characteristics associated with dinitrophenylhydrazone-amino compounds. The infrared spectrum of substance A points to a dinitrophenylhydrazone-amino acid structure.