Expression Cloning of an Adenylate Cyclase-Coupled Calcitonin Receptor
- 15 November 1991
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5034), 1022-1024
- https://doi.org/10.1126/science.1658940
Abstract
A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant Kd approximately 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.Keywords
This publication has 17 references indexed in Scilit:
- Activity-Dependent Synaptic Competition in Vitro: Heterosynaptic Suppression of Developing SynapsesScience, 1991
- Cell Cycle-dependent Coupling of the Calcitonin Receptor to Different G ProteinsScience, 1991
- Multiple sequence alignment of protein families showing low sequence homology: a methodological approach using database pattern-matching discriminators for G-protein-linked receptorsGene, 1991
- Evidence that the action of calcitonin on rat osteoclasts is mediated by two G proteins acting via separate post-receptor pathwaysJournal of Endocrinology, 1990
- Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.Proceedings of the National Academy of Sciences, 1987
- The detection and classification of membrane-spanning proteinsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- G proteins and dual control of adenylate cyclaseCell, 1984
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressinBiochemical and Biophysical Research Communications, 1978
- EFFECTS OF GUANYL NUCLEOTIDES ON CALCITONIN-SENSITIVE ADENYLATE CYCLASE AND CALCITONIN BINDING IN RAT RENAL CORTEXJournal of Endocrinology, 1978