DEVELOPMENT OF AN HPLC METHOD FOR THE TOXICOLOGICAL SCREENING OF CENTRAL NERVOUS SYSTEM DRUGS
- 10 April 2000
- journal article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 23 (7), 1039-1059
- https://doi.org/10.1081/jlc-100101506
Abstract
A simple and sensitive HPLC (high performance liquid chromatography) method has been developed for the qualitative and quantitative analysis of several CNS (central nervous system) drugs in clinical and forensic toxicology. The leading conditions were studied, namely parameters such as mobile phase pH, organic modifier percentage, and salt concentration. An isocratic HPLC elution, using a mobile phase composed of acetonitrile and pH 2.8 aqueous tetramethylammonium perchlorate and a C8 reversed phase column as the stationary phase, was found to be convenient for the separation of several CNS drugs, and for their detection and quantitation. The identification of the drugs was assured using their relative retention times, together with the peak area ratios at two different wavelengths (230 and 270 nm). A quick pre-treatment of the plasma samples, based on a SPE (solid phase extraction) procedure, with good extraction efficiency and satisfactory selectivity was developed. Under these conditions, a mixture of fifteen CNS drugs (including antipsychotics, antidepressants and antiepileptics) and some selected active metabolites, was well separated for identification and quantitative determination purposes.Keywords
This publication has 17 references indexed in Scilit:
- Determination of benzodiazepines in human urine and plasma with solvent modified solid phase micro extraction and gas chromatography; rationalisation of method development using experimental design strategiesJournal of Pharmaceutical and Biomedical Analysis, 1998
- Isocratic High-Performance Liquid Chromatography for the Simultaneous Separation of Eleven Tricyclic Imipramine-Derived Compounds of Therapeutic InterestJournal of Liquid Chromatography & Related Technologies, 1998
- Simultaneous extraction of selected benzodiazepines and benzodiazepine-glucuronides from urine by immunoadsorption.Journal of Analytical Toxicology, 1998
- ClozapineCNS Drugs, 1995
- Use of high-performance liquid chromatography-diode array detection in forensic toxicologyJournal of Chromatography A, 1995
- Reversed-phase high-performance liquid chromatographic database of retention indices and UV spectra of toxicologically relevant substances and its interlaboratory useJournal of Chromatography A, 1994
- Dual ultraviolet wavelength high-performance liquid chromatographic method for the forensic or clinical analysis of seventeen antidepressants and some selected metabolitesJournal of Chromatography B: Biomedical Sciences and Applications, 1993
- Simple and Rapid Screening Procedure for 27 Neuroleptics using HPLC/DADJournal of Liquid Chromatography, 1992
- Rapid Screening for 100 Basic Drugs and Metabolites in Urine Using Cation Exchange Solid-Phase Extraction and High-Performance Liquid Chromatography with Diode Array DetectionJournal of Analytical Toxicology, 1990
- Use of dual-wavelength UV detection in high-performance liquid chromatography for the identification of barbituratesJournal of Chromatography A, 1980