Biochemistry of ATP-dependent red cell membrane shape change
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 41 (sup156), 139-144
- https://doi.org/10.3109/00365518109097446
Abstract
Red cell membranes prepared by hemolysis and washing in hypotonic Tris buffer crenate when suspended at 0°C in isotonic medium. At 37°C, in the presence of 1 mm MgATP, the crenated membranes are progressively converted to smooth-contoured discs and cup-forms. The phosphorylation of proteins and lipids during shape transformation in the presence of [γ-32P]ATP has been studied. Spectrin phosphorylation and shape change could be dissociated in several ways, demonstrating that spectrin phosphorylation is neither necessary nor sufficient for the membrane smoothing reaction. Adenosine markedly inhibited phosphoinositide regeneration without altering shape change. Phos-phatidic acid synthesis from endogenous diacylglycerol was not affected by adenosine and comparison of sheep, human and rabbit ghosts, which vary greatly in shape change capacity, demonstrated a direct correlation between phosphatidic acid synthesis and shape change rate. The results suggest that membrane curvature may be induced by diacylglycerol phosphorylation at the inner surface of the membrane bilayer, while the membrane skeleton limits the curvature and determines the shape ultimately assumed.Keywords
This publication has 19 references indexed in Scilit:
- Rapid transbilayer diffusion of 1,2-diacylglycerol and its relevance to control of membrane curvatureNature, 1978
- A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Phosphorylation and dephosphorylation of spectrinJournal of Supramolecular Structure, 1978
- Properties of human erythrocyte phosphatidylinositol kinase and inhibition by adenosine, ADP and related compoundsBiochimica et Biophysica Acta (BBA) - General Subjects, 1977
- On the mechanism of ATP-induced shape changes in the human erythrocyte membranes: the role of ATPThe Journal of cell biology, 1977
- A solid-liquid composite model of the red cell membraneThe Journal of Membrane Biology, 1976
- Regulation of plasma membrane protein phosphorylation in two mammalian cell typesJournal of Cellular Physiology, 1976
- Rapid breakdown of diphosphoinositide and triphosphoinositide in erythrocyte membranesJournal of Cellular Physiology, 1976
- Localization of enzymes involve in polyphosphoinositide metabolism on the cytoplasmic surface of the human erythrocyte membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971