The Effect of Bonded Ligand Structure on Solute Retention in Reversed-Phase High Performance Liquid Chromatography

Abstract

A comparison of the chromatographic retention characteristics of nonpolar bonded stationary phases that offer a systematic change in bonded ligand geometry was undertaken. The study permits an evaluation of the influence of bonded ligand structure on stationary phase contributions to selectivity in reversed-phase systems. It was found that an n-heptyl bonded phase proved superior in terms of capacity and selectivity to bonded cyclohexyl and bicycloheptyl systems. The cyclohexyl phase, however, displayed significant differential preferability for cyclic hydrocarbon solutes as compared to linear alkane solutes.