Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.
Open Access
- 1 September 1990
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 10 (9), 4987-4989
- https://doi.org/10.1128/mcb.10.9.4987
Abstract
A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.This publication has 21 references indexed in Scilit:
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