An indirect method for quantitation of cellular zinc content of timm-stained cerebellar samples by energy dispersive X-ray microanalysis
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 89 (5), 493-497
- https://doi.org/10.1007/bf00492607
Abstract
The absolute concentration of zinc in the Purkinje cells of the rat cerebellum was determined by means of energy dispersive X-ray microanalysis (EDAX). Gelatine blocks with known zinc concentrations were stained by Timm's sulphide-silver method, and their silver concentrations were measured by EDAX. A linear correlation was found between the zinc and silver concentrations and this linear function was used as a quantitative calibration for evaluation of sulphide-silver staining, after perfusion with sodium-sulphide solution, fixation with glutaraldehyde, cryostat sectioning and staining of cerebellar samples in Timm's reagent.Keywords
This publication has 43 references indexed in Scilit:
- Gelatin Embedding of Central Nervous System Tissue Improves the Quality of Vibratome SectionsStain Technology, 1986
- Cytoarchitectonic distribution of zinc in the hippocampus of man and the ratBrain Research, 1983
- Acid-vapor decomposition for determination of zinc in brain tissue by isotope dilution mass spectrometryAnalytical Chemistry, 1983
- First visualization of glutamate and GABA in neurones by immunocytochemistryNature, 1983
- Histochemical detection of zinc and copper in various neurons of the central nervous systemActa Histochemica, 1981
- Differential effects of GABA analogues and zinc on glutamate decarboxylase, 4‐aminobutyric‐2‐oxoglutaric acid transaminase and succinate semialdehyde dehydrogenase in rat brain tissueJournal of Neurochemistry, 1979
- Energy dispersive X-ray microanalysis of the electrolytes in biological bulk specimen: I. Specimen preparation, beam penetration, and quantitative analysisJournal of Ultrastructure Research, 1977
- The use of thin specimens for X-ray microanalysis in biologyJournal of Microscopy, 1973
- ELECTRON MICROSCOPIC DEMONSTRATION OF ZINC IN THE HIPPOCAMPAL FORMATION USING TIMM'S SULFIDE-SILVER TECHNIQUEJournal of Histochemistry & Cytochemistry, 1969
- TOPOGRAPHIC DETERMINATION OF ZINC IN HUMAN BRAIN BY ATOMIC ABSORPTION SPECTROPHOTOMETRY1Journal of Neurochemistry, 1968