Cytoskeleton-disrupting drugs enhance effect of growth factors and hormones on initiation of DNA synthesis.
- 1 December 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (12), 6435-6438
- https://doi.org/10.1073/pnas.76.12.6435
Abstract
Addition of growth factors such as prostaglandin[PG]F2.alpha. or fibroblastic growth factor [FGF] to quiescent Swiss mouse 3T3 cells resulted in an abrupt increase in the rate of initiation of DNA synthesis after a lag phase of 13-15 h. This increase could be quantified by a rate constant k. Addition of colchicine, Colcemid or vinblastine had a synergistic effect on the initiation of DNA synthesis triggered by PGF2.alpha. or FGF by increasing the value of k. These drugs alone had no effect. Colchicine had a synergistic effect only if added within 8 h of the PGF2.alpha. or FGF addition. Colchicine exerted its full effect when it was present only for the first 5 h with either growth factor. An intact cytoskeleton is not required for the initiation of DNA synthesis. Furthermore, cytoskeleton-disrupting drugs enhance the stimulatory effect of the growth factors.This publication has 15 references indexed in Scilit:
- Action of growth factors in the cell cycleBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1979
- Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cellsProceedings of the National Academy of Sciences, 1978
- Animal Cell CycleAnnual Review of Biochemistry, 1978
- Continuous protein synthesis is required to maintain the probability of entry into S phaseCell, 1977
- Temporal sequence of hormonal interactions during the prereplicative phase of quiescent cultured 3T3 fibroblasts.Proceedings of the National Academy of Sciences, 1977
- Cells regulate theri proliferation through alterations in transition probabilityJournal of Cellular Physiology, 1977
- Role of surface modulating assemblies in growth control of normal and transformed fibroblasts.Proceedings of the National Academy of Sciences, 1977
- Stimulation of DNA synthesis and cell division in a chemically defined medium: Effect of Epidermal Growth Factor, Insulin and Vitamin B12 on resting cultures of 3T6 cellsBiochemical and Biophysical Research Communications, 1976
- Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growthNature, 1974
- QUANTITATIVE STUDIES OF THE GROWTH OF MOUSE EMBRYO CELLS IN CULTURE AND THEIR DEVELOPMENT INTO ESTABLISHED LINESThe Journal of cell biology, 1963