Vesicular-Arbuscular Mycorrhizal Infection in Transformed Root-Inducing T-DNA Roots Grown Axenically

Abstract

Details are given of a technique for obtaining typical vesicular-arbuscular mycorrhizal infections in root organ cultures of Convolvulus sepium transformed by root-inducing T-DNA from Agrobacterium rhizogenes. A biocompartmental culture system was used. Roots growing from a modified Murashige and Skoog nutrient medium and sugar into a compartment containing water agar and neutralized peat, were infected with germinated spores of Glomus mosseae. The uses of such a system for studies of nutrient exchange between plant and fungus are discussed.