Isolation of Uridine 5′-Pyrophosphate Glucuronic Acid Pyrophosphorylase and Its Assay Using 32P-Pyrophosphate
- 1 June 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 59 (6), 1082-1084
- https://doi.org/10.1104/pp.59.6.1082
Abstract
A procedure was devised to detect and assay UDP-glucuronic acid pyrophosphorylase [EC 2.7.7.aa] in plant extracts [Lilium longiflorum]. Substrates are UDP-glucuronic acid and 32P-pyrophosphate, and the 32P-UTP produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine 32P-ATP produced by ADP glucose pyrophosphorylase, and the modification greatly improves the retention of UTP.This publication has 9 references indexed in Scilit:
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