Section preparation of human marrow for light microscopy.
- 1 August 1976
- journal article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 29 (8), 704-710
- https://doi.org/10.1136/jcp.29.8.704
Abstract
Preparation of human marrow sections has been studied systematically in order to facilitate accurate identification of marrow cells. Both of the methods developed involve embedding marrow cores in methyl methacrylate. In one, acrolein fixation is followed by staining of deplasticized sections with eosine-y followed by azure II; in the other, neutrophilic forms are identified by their esterase-specific reactivity in marrow fixed with neutral-buffered formalin. These preparations are suitable for quantitative studies of marrow cellularity.Keywords
This publication has 12 references indexed in Scilit:
- Non-Hodgkin's lymphomas.I. Bone marrow involvementCancer, 1972
- Clinicopathologic studies of 117 untreated patients subjected to laparotomy for the staging of Hodgkin's diseaseCancer, 1971
- Bone marrow biopsy with unaltered architecture: a new biopsy device.1971
- The demonstration of anions generated by the action of sodium hypochlorite (NaOCl) on tissue sections, including observations on the unmasking of carbonyl groupsHistochemistry and Cell Biology, 1969
- An Adaptation of Methacrylate Embedding for Routine Histopathologic UseBlood, 1969
- Präparative Voraussetzungen zur klinischen Histologie des menschlichen KnochenmarksBlut: Zeitschrift für die Gesamte Blutforschung, 1966
- Decalcification for histochemical demonstration of hydrolytic and oxidative enzymesHistochemistry and Cell Biology, 1965
- Needle Biopsy of Bone and MarrowArchives of Internal Medicine, 1964
- A Plastic Embedding Medium for Thin Sectioning in Light MicroscopyStain Technology, 1963
- An adaptation of the Maximow technique for preparation of sections of hematopoietic tissues.1953