Abstract
The ability of a series of compounds to uncouple oxidative phosphorylation or rat-liver mitochondria has been investigated. The compounds were: 2-amino-l,l,3-tricyanopropene; carbonyl cyanide phenylhydrazone and its m-chloro and p-trifluoromethoxy derivatives; 4,5,6,7-tetra-chloro-, 5-chloro-4-nitro-, 5-nitro- and 4,5,6,7-tetrachloro-l-methyl-benzotriazole; 4-hydroxy-3,5-di-iodo-, 3,5-dibromo-4-hydroxy- and 3,5-dichloro-4-hydroxy-benzonitrile; and pentafluorophenol. In a medium the components and physical condition of which were, as far as possible, kept constant, each compound was tested for ability to stimulate adenosine triphosphatase, to stimulate respiration in the presence of pyruvate as substrate, to inhibit phosphate uptake and to prevent swelling by trimethyltin. Each compound was also examined with respect to its ability to produce rapid rigor mortis in mice. The biological properties were compared with the dissociation constant and the hexane -water partition coefficient for each compound. With the exception of 4,5,6,7-tetrachloro-1-methylbenzotriazole, all the compounds behaved qualitatively as 2,4-dinitrophenol. Within each class of compound there is a relation between biological activity and the physical attributes measured. The most efficient uncouplers were the most acidic and the most hydrophobia.