Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Polη
- 12 February 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 283 (5404), 1001-1004
- https://doi.org/10.1126/science.283.5404.1001
Abstract
The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here,RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.Keywords
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