Rat pancreas contains the proglucagon(64–69) fragment and arginine stimulates its release
- 5 August 1985
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 187 (2), 307-310
- https://doi.org/10.1016/0014-5793(85)81265-5
Abstract
Rat proglucagon(64–69) corresponding to the C-terminal hexapeptide of putative rat glicentin sequence in the precursor was synthesized. A glicentin C-terminal hexapeptide specific radioimmunoassay, using the synthetic hexapeptide as standard, demonstrated the presence in rat pancreas of a peptide identified with the synthetic rat proglucagon(64–69): H-Asn-Arg-Asn-Asn-Ile-Ala-OH. The hexapeptide was released concomitantly with glucagon by arginine stimulation from the isolated perfused rat pancreas. The results indicate that the pancreas co-stores and possibly co-releases the hexapeptide with glucagon as one of the processing products of proglucagonKeywords
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