Caki-1 Cells Represent an in vitro Model System for Studying the Human Proximal Tubule Epithelium

Abstract

The human proximal tubule (PT) epithelium is distinguished from other nephron segments via several unique characteristics. Studies assessing PT epithelium increasingly employ cell lines, bypassing the complexity of primary cell cultures. However, few human model systems exist for studying PT cells in vitro. The current work involves an intensive characterization of Caki-1 cells, a commercially available human renal cell line. Caki-1 cells were validated as a representative model system for PT cell research via morphological, physiological and biochemical investigations including light and transmission electron microscopy, transepithelial electrical resistance (TER) measurements and the detection of PT markers. Morphologically, these cells form a polarized monolayer with apical located microvilli and multiple mitochondria per cell. Low TER ranging from 2 to 28 Omega cm(2) was determined for Caki-1 cells, characteristic of the 'leaky' PT epithelium in vivo. Expression of the PT markers: NHE3, GGT, DPP IV, APM and AP were present in Caki-1 cells. Two epithelial markers, E-cadherin and Na(+)/K(+)-ATPase, were additionally observed. The current work is a concise summary which confirms that Caki-1 cells represent well-differentiated polarized PT cells in vitro, regardless of its cancerous origin and multiple passaging. They prove to be a significant contribution to the field of PT research.