Enzymatic Incorporation of ATP and CTP Analogues into the 3' End of tRNA

Abstract

Structural analogues of adenosine 5′‐triphosphate and cytidine 5′‐triphosphate were investigated as substrates for ATP(CTP): tRNA nucleotidyl transferase. Eight out of 26 ATP analogues and six out of nine CTP analogues were incorporated into the 3′ terminus of tRNA. In general, for the recognition of the substrates the modification of the cytidine is less critical than is the modification of adenosine. An isosteric substitution on the ribose residue is possible in both CTP and ATP. The free hydroxyls of these triphosphates can be replaced by an amino group or hydrogen atom without loss of substrate properties. Modifications of positions 1, 2, 6 and 8 on the adenine ring of ATP are not allowed whereas modification on positions 2, 4 and 5 on the cytosine ring of CTP are tolerated by the enzyme. No differences can be observed in the substrate properties of ATP(CTP): tRNA nucleotidyl transferase isolated from different sources. Methods for preparation of tRNA species, which are shortened at their 3′ end by one or more nucleotides, and analytical procedures for characterisation of these modified tRNAs are described.