Facile Preparation of Carbohydrate Microarrays by Site-Specific, Covalent Immobilization of Unmodified Carbohydrates on Hydrazide-Coated Glass Slides
- 24 August 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 7 (19), 4269-4272
- https://doi.org/10.1021/ol051753z
Abstract
A new, simple and efficient immobilization method to attach mono-, di-, oligo-, and polysaccharides to hydrazide-coated glass slides was developed. Protein and cell-binding experiments show that the carbohydrate microarrays prepared by this method are applicable for the rapid analysis of protein−carbohydrate interactions and fast detection of pathogens.Keywords
This publication has 28 references indexed in Scilit:
- Fabrication of Chemical Microarrays by Efficient Immobilization of Hydrazide‐Linked Substances on Epoxide‐Coated Glass SurfacesAngewandte Chemie International Edition, 2005
- Carbohydrate Microarrays: An Advanced Technology for Functional Studies of GlycansChemistry – A European Journal, 2005
- The Use of Carbohydrate Microarrays to Study Carbohydrate-Cell Interactions and to Detect PathogensChemistry & Biology, 2004
- Printed covalent glycan array for ligand profiling of diverse glycan binding proteinsProceedings of the National Academy of Sciences, 2004
- Covalent Display of Oligosaccharide Arrays in Microtiter PlatesJournal of the American Chemical Society, 2004
- Carbohydrate Chips for Studying High-Throughput Carbohydrate−Protein InteractionsJournal of the American Chemical Society, 2004
- Probing Protein–Carbohydrate Interactions with Microarrays of Synthetic OligosaccharidesChemBioChem, 2004
- Intact cell adhesion to glycan microarraysGlycobiology, 2003
- A new kind of carbohydrate array, its use for profiling antiglycan antibodies, and the discovery of a novel human cellulose-binding antibodyGlycobiology, 2003
- Fabrication of Carbohydrate Chips for Studying Protein Carbohydrate InteractionsAngewandte Chemie International Edition, 2002