NITROGEN FIXATION BY CELL-FREE PREPARATIONS FROM MICROORGANISMS
Open Access
- 1 May 1960
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 46 (5), 726-733
- https://doi.org/10.1073/pnas.46.5.726
Abstract
Cell-free N-fixation has been achieved with preparations from Cloatridium pasteurianum, Rhodospirillum rubrum, and several species of blue-green algae. The observations of Carnahan et al. with C. pasteurianum have been fully verified. The fixation by the preparations from this organism is coupled to the metabolism of pyruvate. The isolation of free ammonia, containing up to 52 atom percent N15 excess, from the reaction mixtures provides support for the ammonia hypothesis of N-fixation. This communication appears to be the first report of substantial cell-free fixation of N by Rhodospirillum rubrum and by blue-green algae.This publication has 7 references indexed in Scilit:
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