Persistent rat parvovirus infection in individually housed rats
- 1 September 1991
- journal article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 117 (3-4), 193-205
- https://doi.org/10.1007/bf01310765
Abstract
The duration of infection with rat virus (RV), an autonomous rodent parvovirus, was examined at multiple intervals over 6 months in rats inoculated by the oronasal route at 2 days of age or 4 weeks of age and individually housed after weaning to prevent cross-infection. Infectious virus was recovered by explant culture from 32 of 80 rats inoculated as pups and was detected as late as 6 months after inoculation. Rats inoculated as juveniles developed acute infection, but virus was not detected beyond 7 weeks after inoculation. Tissues from rats in both age groups were surveyed for RV DNA by Southern blotting using a double-stranded DNA probe made from a 1700 bp cloned fragment of RV spanning map units 0.19–0.52. Band patterns representative of acute infection (juvenile rats) were consistent with the replicating form of RV DNA, whereas patterns representative of persistent infection (rats inoculated as pups) were suggestive of defective or non-productive viral replication.Keywords
This publication has 31 references indexed in Scilit:
- Modulation of lethal and persistent rat parvovirus infection by antibodyArchiv für die gesamte Virusforschung, 1991
- Persistence of parvovirus H-1 DNA in human B- and T-lymphoma cellsVirus Research, 1990
- Analysis of parvovirus infections using strand-specific hybridization probesVirus Research, 1989
- Persistence of rat parvovirus in athymic ratsArchiv für die gesamte Virusforschung, 1989
- The pathogenesis of rat virus infection in infant and juvenile rats after oronasal inoculationArchiv für die gesamte Virusforschung, 1987
- Persistence of rat virus in seropositive rats as detected by explant cultureArchiv für die gesamte Virusforschung, 1987
- Electron microscopic comparison of the sequences of single-stranded genomes of mammalian parvoviruses by heteroduplex mappingJournal of Molecular Biology, 1983
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967
- Viral Hemorrhagic Encephalopathy of RatsScience, 1967