Synaptophysin: a marker protein for neuroendocrine cells and neoplasms.
- 1 May 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (10), 3500-3504
- https://doi.org/10.1073/pnas.83.10.3500
Abstract
Synaptophysin is an integral membrane glycoprotein (Mr 38,000) that occurs in presynaptic vesicles of neurons and in similar vesicles of the adrenal medulla. By using a monoclonal antibody to this protein (SY38), we have found, by immunohistochemistry and immunoblotting, that an identical or similar protein is also expressed in neuroendocrine tumors of neural type, such as pheochromocytomas and paragangliomas. In addition, this protein occurs in certain neuroendocrine epithelial cells, such as pancreatic islet cells; in a variety of neuroendocrine epithelial tumors, including islet-cell adenomas and carcinomas and several carcinoids and neuroendocrine carcinomas of the gastrointestinal and the bronchial tracts; and in medullary carcinomas of the thyroid. Our results show that synaptophysin, and the vesicles that contain it, can occur in normal and neoplastic neuroendocrine cells of natural type, as demonstrated by colocalization with neurofilaments, as well as in those of epithelial type, as shown by colocalization with cytokeratin filaments and desmoplakins. We conclude that synaptophysin is expressed independently of other neuronal diffentiation markers and propose that it be used as a differentiation marker in tumor diagnosis.This publication has 31 references indexed in Scilit:
- Secretogranins I and II: two tyrosine-sulfated secretory proteins common to a variety of cells secreting peptides by the regulated pathway.The Journal of cell biology, 1985
- Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesiclesCell, 1985
- A dual expression of cytokeratin and neurofilaments in bronchial carcinoid cellsInternational Journal of Cancer, 1985
- Chromogranin immunoreactivity in the central nervous system. Immunochemical characterisation, distribution and relationship to catecholamine and enkephalin pathwaysBrain Research Reviews, 1984
- Identification of Merkel cells in human skin by specific cytokeratin antibodies:Differentiation, 1984
- The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cellsCell, 1982
- Diversity of cytokeratinsJournal of Molecular Biology, 1981
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981
- Viral transformation of chick myogenic cellsExperimental Cell Research, 1979
- THE CYTOCHEMISTRY AND ULTRASTRUCTURE OF POLYPEPTIDE HORMONE-PRODUCING CELLS OF THE APUD SERIES AND THE EMBRYOLOGIC, PHYSIOLOGIC AND PATHOLOGIC IMPLICATIONS OF THE CONCEPTJournal of Histochemistry & Cytochemistry, 1969