Purification and characterization of a 32‐kDa phospholipase A2 inhibitory protein (lipocortin) from human peripheral blood mononuclear cells
- 13 July 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 219 (1), 169-175
- https://doi.org/10.1016/0014-5793(87)81211-5
Abstract
A 32-kDa protein was isolated from human monocytes after calcium precipitation and chromatography. The protein activity was assessed by the inhibition of soluble phospholipase A2, (PLA2). This in vitro inhibitory effect on phospholipases A2 was found only with negatively charged phospholipids. The protein was also able to inhibit cellular PLA2, in mouse thymocytes. The biochemical properties and amino acid composition strongly suggest that the protein shares similarities with endonexin. Using a neutralizing monoclonal antibody against rat lipocortin, we found a cross-reactivity with the 32-kDa protein. According to the biochemical and immunological properties, we propose to relate this PLA2, inhibitory protein from human monocytes to lipocortin.Keywords
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