Autoradiographic demonstration of DNA repair synthesis in ganglion cells of aquarium fish at various age in vivo

Abstract

Summary Unscheduled DNA synthesis in highly differentiated ganglion cells of adult medaka (Oryzias latipes) was demonstrated by autoradiography. For this, part of bony skull of the fish was surgically removed and the brain of the living fish was directly exposed to a solution of a carcinogen and³H-TdR. This technique is suitable for precise measurement of DNA repair synthesis in non-dividing cells of the central nervous system in vivo. Using this system, the effect of aging on the levels of DNA repair was investigated. There was no age-associated change in the ability of repair in ganglion cells of the medaka of various ages.