DNA-fluorometry of mammalian sperm
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 54 (1), 1-7
- https://doi.org/10.1007/bf00493324
Abstract
The DNA-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescence distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature sperm with a coefficient of variation below 2%.Keywords
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