Applicability of the Popliteal Lymph Node Assay in the Brown-Norway Rat
- 1 January 1990
- journal article
- Published by Taylor & Francis in Immunopharmacology and Immunotoxicology
- Vol. 12 (4), 669-677
- https://doi.org/10.3109/08923979009019683
Abstract
A number of drugs and chemicals can induce autoimmune disorders. Previous studies suggested the popliteal lymph node (PLN) assay might be a suitable and reliable model to detect such compounds in the mouse. the applicability of this assay has been examined in the Brown-Norway rat using a pannel of positive as well as negative reference compounds. Popliteal lymph nodes were excised and weighed seven days after subcutaneous injection of 5 mg of each compound into one footpad. Interestingly, all negative reference compounds produced no significant increase in PLN weight index whereas four positive reference compounds were associated with PLN-weight indices greater than 2.3. However, no increase in PLN weight was noted in procainamide-and isoniazide-treated rats as previously reported in the mouse. This first set of experiments indicates that the PLN assay is applicable to Brown-Norway rats and warrants further investigation.Keywords
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