Release and Synthesis of Luteinizing Hormone and Follicle-Stimulating Hormone in Pituitary Cultures in Response to Hypothalamic Preparations

Abstract

Addition of microgram amounts of highly purified porcine LH-RH twice daily for 3 days to female rat anterior pituitaries in vitro significantly increased the quantities of LH and FSH released. Total LH and FSH contents in stimulated tissue and medium exceeded those of controls as measured by radioimmunoassays for LH and FSH and by bioassay for FSH.