Cell‐Wall Lipopolysaccharide of Escherichia coli O114:H2

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Abstract
The O-specific polysaccharide of the 0114 antigen (lipopolysaccharide) of E. coli 0114 and oligosaccharides obtained from it by Smith degradation and hydrogen fluoride solvolysis were analyzed, using proton and 13C NMR spectroscopy and methylation. The 0114 polysaccharide has the tetrasaccharide repeating unit .alpha.-N-acetylglucosamine(1 .fwdarw. 4).beta.-3,6-dideoxy-3-(N-acetyl-L-seryl)aminoglucose(1 .fwdarw. 3).beta.-ribofuranose(1 .fwdarw. 4)galactose. In the polysaccharide the repeating units are joined through .beta.1 .fwdarw. 3-galactosyl linkages. This structure is compared with that of the serologically cross-reacting Shigella boydii 08 antigen and the serological similarity is discussed.