Production and characterization of a monoclonal antibody to chicken type I collagen.
- 1 August 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (8), 3703-3707
- https://doi.org/10.1073/pnas.76.8.3703
Abstract
Lymphocyte-myeloma [mouse] cell hybridization can be used to produce large amounts of extremely high-titer specific antibodies against type I collagen, a macromolecule normally of low immunogenicity. In a passive hemagglutination assay the antibody had a high titer against chicken type I collagen but showed no activity against chicken type II or rat type I collagen. By using a 2-step fluorescence histochemical procedure on sections of embryonic chicken tibia, strong fluorescence was observed in the perichondrium and surrounding connective tissue (known to contain type I collagen) but not over the cartilage (characterized by type II collagen). When used in conjunction with S. aureus as a solid phase immunoadsorbant, the antibody was shown to bind to labeled collagen synthesized in vitro by embryonic chicken calvaria.This publication has 23 references indexed in Scilit:
- Assignment of a type I collagen structural gene to human chromosome 7Nature, 1978
- A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cellsSomatic Cell and Molecular Genetics, 1977
- Somatic cell hybridization of mouse myeloma cellsCell, 1976
- Study of differential collagen synthesis during development of the chick embryo by immunofluorescence: I. Preparation of collagen type I and type II specific antibodies and their application to early stages of the chick embryoDevelopmental Biology, 1976
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Isolation and partial characterization of procollagen fractions produced by a clonal strain of calf dermatosparactic cellsJournal of Molecular Biology, 1974
- Temporal and spatial transitions in collagen types during embryonic chick limb developmentDevelopmental Biology, 1973
- Structure of rat skin collagen α1-CB8. Amino acid sequence of the hydroxylamine-produced fragment HA2Biochemistry, 1972
- Isolation and characterization of a chick cartilage collagen containing three identical chainsBiochemistry, 1971
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964