Comparison of histones in normal and xeroderma pigmentosum lymphoblastoid cells

Abstract

Histones from normal human and xeroderma pigmentosum, complementation group A (XPA), lymphoblastoid cells were compared both quantitatively, qualitatively and for binding affinity for DNA. Electrophoretic examination of the histones showed that all five major histone species were present in both cell groups and that there were no quantitative differences between normal and XPA histones. Binding affinity to [3H] mammalian DNA of the histones was determined. No significant differences were observed in binding of either normal or XPA histones to DNA.