Effect of Cortisol on Amino Acid Incorporation by Nuclear and Cytoplasmic Fractions of Rabbit Thymus
- 1 February 1968
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 82 (2), 277-281
- https://doi.org/10.1210/endo-82-2-277
Abstract
Thymi were removed from rabbits at 4, 6 and 12 hr. after injection of cortisol. Nuclei, microsomal, ribosomal and the pH 5.1 fractions were prepared and the in vitro incorporation of leucine-l-14c was determined and compared with the activity of similar fractions from control tissue. The activity of all fractions was inhibited at 4 hr. inhibition being greatest in the microsomal fraction. Analysis of cytoplasmic fractions for nuclear breakdown products indicated only small amounts of DNA in preparations from treated tissue 4 hr. after cortisol. At 12 hr. after cortisol microsomal preparations contained 28% DNA; lesser amounts were found in other fractions.This publication has 11 references indexed in Scilit:
- Alterations in Protein and Nucleic Acid Metabolism of Thymocytes Produced by Adrenal Steroids in VitroJournal of Biological Chemistry, 1966
- Acute decrease in RNA polymerase activity of rat thymus in response to cortisol injection.Proceedings of the National Academy of Sciences, 1966
- A Time Study on the Effect of Cortisol on the Incorporation of Thymidine-2-14C Into Nucleic Acids of Mouse Lymphatic TissueEndocrinology, 1966
- Effect of hydrocortisone on amino acid incorporation by microsomes isolated from mouse lymphoma ML-388 cells and rat thymusBiochemical Pharmacology, 1964
- Effect of X-irradiation on amino acid incorporation into thymus cell fractionsArchives of Biochemistry and Biophysics, 1964
- Effect of Irradiation and Cortisol on Thymus Fraction.Experimental Biology and Medicine, 1962
- GROWTH INHIBITORY EFFECTS OF HYDROCORTISONE ON MOUSE LYMPHOMA ML-388 IN-VITRO1962
- Intermediate reactions in protein biosynthesisBiochimica et Biophysica Acta, 1957
- A SENSITIVE METHOD FOR THE DETERMINATION OF RIBONUCLEIC ACID IN TISSUES AND MICROORGANISMSJournal of Biological Chemistry, 1956
- A SENSITIVE METHOD FOR THE DETERMINATION OF DEOXYRIBONUCLEIC ACID IN TISSUES AND MICROORGANISMSJournal of Biological Chemistry, 1955